Waiczies, Sonia


Scientist, Berlin Ultrahigh Field Facility (B.U.F.F.), Max-Delbrueck Center for Molecular Medicine, Berlin, Germany
+49 (0) 30 9406 4542

Our group uses magnetic resonance imaging (MRI) to study inflammation and anti-inflammatory drugs in vivo. We seek to better understand the kinetics and dynamics of immune cell invasion during inflammation within the central nervous system (CNS). For instance, brain inflammation in multiple sclerosis involves immune-cell recruitment during the early pathogenic stages before clinical symptoms appear. The blood-brain barrier (BBB) restricts migration of immune cells to the CNS, but during inflammation the BBB is altered.

Fluorine imaging: We employ fluorine MR methods to study inflammation and anti-inflammatory drugs in vivo. We label immune cells with fluorine (19F) for MRI detection. Immune cells are labeled with perfluorocarbon (PFC)-rich particles and are then injected in vivo to be tracked by 19F MRI. 19F MR images are co-registered with conventional proton (1H) MRI. Detection of contrast-enhancing lesions by MRI at the site of BBB disruption with contrast agents such as gadolinium permits correlating the disruption with active inflammation. However, gadolinium extravasation does not provide direct evidence of immune cell CNS trafficking and may occur independent of lesion formation. We are developing novel MRI techniques to gain a more accurate and comprehensive view of CNS inflammation.

To make fluorine MRI possible even in situations where the availability of fluorine is very low, we employ various strategies that boost signal-to-noise ratio (SNR). One strategy is to increase the availability of fluorine e.g. increasing the uptake of fluorine rich nanoparticles by immune cells. Another strategy is use higher magnetic field strengths. We are cooperating with the US National High Magnetic Field Laboratory (MagLab), which is supported by the State of Florida and the National Science Foundation to perform 19F MR measurements. By using cryogenically-cooled coils we can also boost SNR and be able to perform MRI microscopy. Cryogenically-cooled coils are made of superconducting material with decreased coil resistance and thermal noise and thereby increased SNR. Micro MRI enables imaging with a spatial resolution less than 100µm. These coils provide detail in anatomical morphological changes (1H MRI) as well as inflammatory information (19F MRI).


Niendorf T, Waiczies H, Waiczies S. Method for localisation and/or quantification of 19F-containing compounds via 19F magnetic resonance (MR) analysis (EP 13161948.8 2012)

Cantow K, Gladytz T, Millward JM, Waiczies S, Niendorf T, Seeliger E
Monitoring kidney size to interpret MRI-based assessment of renal oxygenation in acute pathophysiological scenarios.
Acta Physiol (Oxf). 2022;:e13868 - PMID: 35993768
Delgado PR, Kuehne A, Aravina M, Millward JM, Vázquez A, Starke L, Waiczies H, Pohlmann A, Niendorf T, Waiczies S
B1 inhomogeneity correction of RARE MRI at low SNR: Quantitative in vivo 19 F MRI of mouse neuroinflammation with a cryogenically-cooled transceive surface radiofrequency probe.
Magn Reson Med. 2022;87(4):1952-1970 - PMID: 34812528
Prinz C, Starke L, Ramspoth TF, Kerkering J, Martos Riaño V, Paul J, Neuenschwander M, Oder A, Radetzki S, Adelhoefer S, Ramos Delgado P, Aravina M, Millward JM, Fillmer A, Paul F, Siffrin V, von Kries JP, Niendorf T, Nazaré M, Waiczies S
Pentafluorosulfanyl (SF5) as a Superior 19F Magnetic Resonance Reporter Group: Signal Detection and Biological Activity of Teriflunomide Derivatives.
ACS Sens. 2021;6(11):3948-3956 - PMID: 34666481 - PMCID: PMC8630787
Periquito JS, Gladytz T, Millward JM, Delgado PR, Cantow K, Grosenick D, Hummel L, Anger A, Zhao K, Seeliger E, Pohlmann A, Waiczies S, Niendorf T
Continuous diffusion spectrum computation for diffusion-weighted magnetic resonance imaging of the kidney tubule system.
Quant Imaging Med Surg. 2021;11(7):3098-3119 - PMID: 34249638 - PMCID: PMC8250037
Gladytz T, Millward JM, Cantow K, Hummel L, Zhao K, Flemming B, Periquito JS, Pohlmann A, Waiczies S, Seeliger E, Niendorf T
Reliable kidney size determination by magnetic resonance imaging in pathophysiological settings.
Acta Physiol (Oxf). 2021;233(2):e13701 - PMID: 34089569
Wachsmuth L, Mensen A, Barca C, Wiart M, Tristão-Pereira C, Busato A, Waiczies S, Himmelreich U, Millward JM, Reimann HM, Jelescu I, Marzola P, Pradier B, Viola A, Faber C
Contribution of preclinical MRI to responsible animal research: living up to the 3R principle.
MAGMA. 2021;34(4):469-474 - PMID: 34009521 - PMCID: PMC8338837
Herrmann CJJ, Els A, Boehmert L, Periquito J, Eigentler TW, Millward JM, Waiczies S, Kuchling J, Paul F, Niendorf T
Simultaneous T2 and T2 mapping of multiple sclerosis lesions with radial RARE-EPI.
Magn Reson Med. 2021;86(3):1383-1402 - PMID: 33951214
Waiczies S, Els A, Kuchling J, Markenroth Bloch K, Pankowska A, Waiczies H, Herrmann C, Chien C, Finke C, Paul F, Niendorf T
Magnetic Resonance Imaging of Multiple Sclerosis at 7.0 Tesla.
J Vis Exp. 2021;(168):ePub - PMID: 33682856
Prinz C, Starke L, Millward JM, Fillmer A, Delgado PR, Waiczies H, Pohlmann A, Rothe M, Nazaré M, Paul F, Niendorf T, Waiczies S
In vivo detection of teriflunomide-derived fluorine signal during neuroinflammation using fluorine MR spectroscopy.
Theranostics. 2021;11(6):2490-2504 - PMID: 33456555 - PMCID: PMC7806491
Millward JM, Ramos Delgado P, Smorodchenko A, Boehmert L, Periquito J, Reimann HM, Prinz C, Els A, Scheel M, Bellmann-Strobl J, Waiczies H, Wuerfel J, Infante-Duarte C, Chien C, Kuchling J, Pohlmann A, Zipp F, Paul F, Niendorf T, Waiczies S
Transient enlargement of brain ventricles during relapsing-remitting multiple sclerosis and experimental autoimmune encephalomyelitis.
JCI Insight. 2020;5(21):ePub - PMID: 33148886 - PMCID: PMC7710287
Cantow K, Hummel L, Flemming B, Waiczies S, Niendorf T, Seeliger E
Imagine physiology without imaging.
Acta Physiol (Oxf). 2020;230(3):e13549 - PMID: 32852085
Delgado PR, Kuehne A, Periquito JS, Millward JM, Pohlmann A, Waiczies S, Niendorf T
B1 inhomogeneity correction of RARE MRI with transceive surface radiofrequency probes.
Magn Reson Med. 2020;84(5):2684-2701 - PMID: 32447779
Niendorf T, Seeliger E, Cantow K, Flemming B, Waiczies S, Pohlmann A
Probing renal blood volume with magnetic resonance imaging.
Acta Physiol (Oxf). 2020;228(4):e13435 - PMID: 31876349
Wang S, Millward JM, Hanke-Vela L, Malla B, Pilch K, Gil-Infante A, Waiczies S, Mueller S, Boehm-Sturm P, Guo J, Sack I, Infante-Duarte C
MR Elastography-Based Assessment of Matrix Remodeling at Lesion Sites Associated With Clinical Severity in a Model of Multiple Sclerosis.
Front Neurol. 2020;10:1382 - PMID: 31998225 - PMCID: PMC6970413
Periquito JDS, Paul K, Huelnhagen T, Ku MC, Ji Y, Cantow K, Gladytz T, Grosenick D, Flemming B, Seeliger E, Waiczies S, Niendorf T, Pohlmann A
Diffusion-weighted Renal MRI at 9.4 Tesla Using RARE to Improve Anatomical Integrity.
Sci Rep. 2019;9(1):19723 - PMID: 31873155 - PMCID: PMC6928203
Starke L, Pohlmann A, Prinz C, Niendorf T, Waiczies S
Performance of compressed sensing for fluorine-19 magnetic resonance imaging at low signal-to-noise ratio conditions.
Magn Reson Med. 2020;84(2):592-608 - PMID: 31863516
Boehmert L, Waiczies H, Kuehne A, Oezerdem C, Waiczies S, Starke L, Ku MC, Pohlmann A, Seeliger E, Niendorf T
Cardiorenal sodium MRI in small rodents using a quadrature birdcage volume resonator at 9.4 T.
MAGMA. 2020;33(1):121-130 - PMID: 31797228
Waiczies S, Srinivas M, Flögel U, Boehm-Sturm P, Niendorf T
Special issue on fluorine-19 magnetic resonance: technical solutions, research promises and frontier applications.
MAGMA. 2019;32(1):1-3 - PMID: 30730025
Prinz C, Delgado PR, Eigentler TW, Starke L, Niendorf T, Waiczies S
Toward 19F magnetic resonance thermometry: spin-lattice and spin-spin-relaxation times and temperature dependence of fluorinated drugs at 9.4 T.
MAGMA. 2019;32(1):51-61 - PMID: 30515642
Waiczies S, Rosenberg JT, Kuehne A, Starke L, Delgado PR, Millward JM, Prinz C, Dos Santos Periquito J, Pohlmann A, Waiczies H, Niendorf T
Fluorine-19 MRI at 21.1 T: enhanced spin-lattice relaxation of perfluoro-15-crown-5-ether and sensitivity as demonstrated in ex vivo murine neuroinflammation.
MAGMA. 2019;32(1):37-49 - PMID: 30421250 - PMCID: PMC6514110
Millward JM, Ariza de Schellenberger A, Berndt D, Hanke-Vela L, Schellenberger E, Waiczies S, Taupitz M, Kobayashi Y, Wagner S, Infante-Duarte C
Application of Europium-Doped Very Small Iron Oxide Nanoparticles to Visualize Neuroinflammation with MRI and Fluorescence Microscopy.
Neuroscience. 2019;403:136-144 - PMID: 29273325
Niendorf T, Pohlmann A, Reimann HM, Waiczies H, Peper E, Huelnhagen T, Seeliger E, Schreiber A, Kettritz R, Strobel K, Ku MC, Waiczies S
Advancing Cardiovascular, Neurovascular, and Renal Magnetic Resonance Imaging in Small Rodents Using Cryogenic Radiofrequency Coil Technology.
Front Pharmacol. 2015;6:255 - PMID: 26617515 - PMCID: PMC4642111
Feutlinske F, Browarski M, Ku MC, Trnka P, Waiczies S, Niendorf T, Stallcup WB, Glass R, Krause E, Maritzen T
Stonin1 mediates endocytosis of the proteoglycan NG2 and regulates focal adhesion dynamics and cell motility.
Nat Commun. 2015;6:8535 - PMID: 26437238 - PMCID: PMC4600748
Ji Y, Waiczies H, Winter L, Neumanova P, Hofmann D, Rieger J, Mekle R, Waiczies S, Niendorf T
Eight-channel transceiver RF coil array tailored for ¹H/¹?F MR of the human knee and fluorinated drugs at 7.0 T.
NMR Biomed. 2015;28(6):726-37 - PMID: 25916199
Ji Y, Waiczies H, Winter L, Neumanova P, Hofmann D, Rieger J, Mekle R, Waiczies S, Niendorf T
Eight-channel transceiver RF coil array tailored for 1H/19F MR of the human knee and fluorinated drugs at 7.0 T
NMR in Biomedicine . 2015;:ePub
Waiczies S, Lepore S, Sydow K, Drechsler S, Ku MC, Martin C, Lorenz D, Schütz I, Reimann HM, Purfürst B, Dieringer MA, Waiczies H, Dathe M, Pohlmann A, Niendorf T
Anchoring dipalmitoyl phosphoethanolamine to nanoparticles boosts cellular uptake and fluorine-19 magnetic resonance signal.
Sci Rep. 2015;5:8427 - PMID: 25673047
Niendorf T, Pohlmann A, Arakelyan K, Flemming B, Cantow K, Hentschel J, Grosenick D, Ladwig M, Reimann H, Klix S, Waiczies S, Seeliger E
How Bold is Blood Oxygenation Level Dependent (BOLD) Magnetic Resonance Imaging of the Kidney? Opportunities, Challenges and Future Directions.
Acta Physiol (Oxf). 2014;:ePub - PMID: 25204811
Pohlmann A, Karczewski P, Ku MC, Dieringer B, Waiczies H, Wisbrun N, Kox S, Palatnik I, Reimann HM, Eichhorn C, Waiczies S, Hempel P, Lemke B, Niendorf T, Bimmler M
Cerebral blood volume estimation by ferumoxytol-enhanced steady-state MRI at 9.4?T reveals microvascular impact of ?1 -adrenergic receptor antibodies.
NMR in Biomedicine . 2014;27(9):1085-93 - PMID: 25060359
Leuenberger T, Pfueller CF, Luessi F, Bendix I, Paterka M, Prozorovski T, Treue D, Luenstedt S, Herz J, Siffrin V, Infante-Duarte C, Zipp F, Waiczies S
Modulation of dendritic cell immunobiology via inhibition of 3-hydroxy-3-methylglutaryl-CoA (HMG-CoA) reductase.
PLoS One. 2014;9(7):e100871 - PMID: 25013913 - PMCID: PMC4094470
Graessl A, Muhle M, Schwerter M, Rieger J, Oezerdem C, Santoro D, Lysiak D, Winter L, Hezel F, Waiczies S, Guthoff RF, Falke K, Hosten N, Hadlich S, Krueger PC, Langner S, Stachs O, Niendorf T
Ophthalmic magnetic resonance imaging at 7 T using a 6-channel transceiver radiofrequency coil array in healthy subjects and patients with intraocular masses.
Investigative Radiology . 2014;49(5):260-70 - PMID: 24651662
Pohlmann A, Arakelyan K, Hentschel J, Cantow K, Flemming B, Ladwig M, Waiczies S, Seeliger E, Niendorf T
Detailing the Relation Between Renal T2* and Renal Tissue pO2 Using an Integrated Approach of Parametric Magnetic Resonance Imaging and Invasive Physiological Measurements.
Investigative Radiology . 2014;:ePub - PMID: 24651661
Vinnakota K, Hu F, Ku MC, Georgieva PB, Szulzewsky F, Pohlmann A, Waiczies S, Waiczies H, Niendorf T, Lehnardt S, Hanisch UK, Synowitz M, Markovic DS, Wolf SA, Glass R, Kettenmann H
Toll-like receptor 2 mediates microglia/brain macrophage MT1-MMP expression and glioma expansion
Neuro-Oncology. 2013;:ePub - EDOC: /13344/
Lepore S, Waiczies H, Hentschel J, Ji Y, Skodowski J, Pohlmann A, Millward JM, Paul F, Wuerfel J, Niendorf T, Waiczies S
Enlargement of cerebral ventricles as an early indicator of encephalomyelitis
PLoS One. 2013;8 (8):e72841 - EDOC: /13248/
Waiczies H, Guenther M, Skodowski J, Lepore S, Pohlmann A, Niendorf T, Waiczies S
Monitoring dendritic cell migration using 19F / 1H magnetic resonance imaging
Journal of Visualized Experiments. 2013;(73):e50251 - EDOC: /12991/
Pohlmann A, Hentschel J, Fechner M, Hoff U, Bubalo G, Arakelyan K, Cantow K, Seeliger E, Flemming B, Waiczies H, Waiczies S, Schunck WH, Dragun D, Niendorf T
High temporal resolution parametric MRI monitoring of the initial ischemia/reperfusion phase in experimental acute kidney injury
PLoS One. 2013;8 (2):e57411 - EDOC: /12950/
Waiczies H, Lepore S, Drechsler S, Qadri F, Purfuerst B, Sydow K, Dathe M, Kuehne A, Lindel T, Hoffmann W, Pohlmann A, Niendorf T, Waiczies S
Visualizing brain inflammation with a shingled-leg radio-frequency head probe for (19)f/(1)h MRI
Scientific Reports. 2013;:1280 - EDOC: /12897/
Ku MC, Wolf SA, Respondek D, Matyash V, Pohlmann A, Waiczies S, Waiczies H, Niendorf T, Synowitz M, Glass R, Kettenmann H
GDNF mediates glioblastoma-induced microglia attraction but not astrogliosis
Acta Neuropathologica. 2013;:ePub - EDOC: /12810/
Hezel F, Thalhammer C, Waiczies S, Schulz-Menger J, Niendorf T
High spatial resolution and temporally resolved t(2) (*) mapping of normal human myocardium at 7.0 tesla: an ultrahigh field magnetic resonance feasibility study
PLoS One. 2012;7 (12):e52324 - EDOC: /12746/
Niendorf T, Graessl A, Thalhammer C, Dieringer MA, Kraus O, Santoro D, Fuchs K, Hezel F, Waiczies S, Ittermann B, Winter L
Progress and promises of human cardiac magnetic resonance at ultrahigh fields: a physics perspective.
Journal of Magnetic Resonance. 2012;229:208-22 - PMID: 23290625
Dieringer B, Pohlmann A, Dieringer MA, Els A, Waiczies H, Waiczies S, Schulz-Menger J, Niendorf T
Functional and morphological cardiac magnetic resonance imaging of mice using a cryogenic quadrature radiofrequency coil
PLoS One. 2012;7 (8):e42383 - EDOC: /12510/
Waiczies H, Millward JM, Lepore S, Infante-Duarte C, Pohlmann A, Niendorf T, Waiczies S,
Identification of cellular infiltrates during early stages of brain inflammation with magnetic resonance microscopy
PLoS One. 2012;7 (3):e32796 - EDOC: /12287/
Waiczies H, Lepore S, Janitzek N, Hagen U, Seifert F, Ittermann B, Purfuerst B, Pezzutto A, Paul F, Niendorf T, Waiczies S
Perfluorocarbon particle size influences magnetic resonance signal and immunological properties of dendritic cells
PLoS One. 2011;6 (7):e21981 - EDOC: /11787/
Waiczies S, Waiczies H
Understanding the pathogenesis of neuroinflammation using magnetic resonance imaging
Malta Medical Journal. 2011;23 (3):56 - EDOC: /13188/
Paul F, Waiczies S, Wuerfel J, Bellmann-Strobl J, Doerr J, Waiczies H, Haertle M, Wernecke KD, Volk HD, Aktas O, Zipp F
Oral high-dose atorvastatin treatment in relapsing-remitting multiple sclerosis
PLoS One. 2008;3 (4):e1928 - EDOC: /9481/
Waiczies S, Bendix I, Zipp F
Geranylgeranylation but not GTP-loading of Rho GTPases determines T cell function
Science Signaling. 2008;1 (12):pt3 - EDOC: /13905/
Waiczies S, Bendix I, Prozorovski T, Ratner M, Nazarenko I, Pfueller CF, Brandt AU, Herz J, Brocke S, Ullrich O, Zipp F
Geranylgeranylation but not GTP loading determines rho migratory function in T cells
Journal of Immunology. 2007;179 (9):6024-6032 - EDOC: /8967/