Scientist, Berlin Ultrahigh Field Facility (B.U.F.F.), Max-Delbrueck Center for Molecular Medicine, Berlin, Germany
+49 (0) 30 9406 4542
Our group uses magnetic resonance imaging (MRI) to study inflammation and anti-inflammatory drugs in vivo. We seek to better understand the kinetics and dynamics of immune cell invasion during inflammation within the central nervous system (CNS). For instance, brain inflammation in multiple sclerosis involves immune-cell recruitment during the early pathogenic stages before clinical symptoms appear. The blood-brain barrier (BBB) restricts migration of immune cells to the CNS, but during inflammation the BBB is altered.
Fluorine imaging: We employ fluorine MR methods to study inflammation and anti-inflammatory drugs in vivo. We label immune cells with fluorine (19F) for MRI detection. Immune cells are labeled with perfluorocarbon (PFC)-rich particles and are then injected in vivo to be tracked by 19F MRI. 19F MR images are co-registered with conventional proton (1H) MRI. Detection of contrast-enhancing lesions by MRI at the site of BBB disruption with contrast agents such as gadolinium permits correlating the disruption with active inflammation. However, gadolinium extravasation does not provide direct evidence of immune cell CNS trafficking and may occur independent of lesion formation. We are developing novel MRI techniques to gain a more accurate and comprehensive view of CNS inflammation.
To make fluorine MRI possible even in situations where the availability of fluorine is very low, we employ various strategies that boost signal-to-noise ratio (SNR). One strategy is to increase the availability of fluorine e.g. increasing the uptake of fluorine rich nanoparticles by immune cells. Another strategy is use higher magnetic field strengths. We are cooperating with the US National High Magnetic Field Laboratory (MagLab), which is supported by the State of Florida and the National Science Foundation to perform 19F MR measurements. By using cryogenically-cooled coils we can also boost SNR and be able to perform MRI microscopy. Cryogenically-cooled coils are made of superconducting material with decreased coil resistance and thermal noise and thereby increased SNR. Micro MRI enables imaging with a spatial resolution less than 100µm. These coils provide detail in anatomical morphological changes (1H MRI) as well as inflammatory information (19F MRI).
Niendorf T, Waiczies H, Waiczies S. Method for localisation and/or quantification of 19F-containing compounds via 19F magnetic resonance (MR) analysis (EP 13161948.8 2012)
Advancing Cardiovascular, Neurovascular, and Renal Magnetic Resonance Imaging in Small Rodents Using Cryogenic Radiofrequency Coil Technology.
Stonin1 mediates endocytosis of the proteoglycan NG2 and regulates focal adhesion dynamics and cell motility.
Eight-channel transceiver RF coil array tailored for ¹H/¹?F MR of the human knee and fluorinated drugs at 7.0 T.
NMR Biomed. 2015;28(6):726-37 - PMID: 25916199
Eight-channel transceiver RF coil array tailored for 1H/19F MR of the human knee and fluorinated drugs at 7.0 T
NMR in Biomedicine . 2015;:ePub
Anchoring dipalmitoyl phosphoethanolamine to nanoparticles boosts cellular uptake and fluorine-19 magnetic resonance signal.
How Bold is Blood Oxygenation Level Dependent (BOLD) Magnetic Resonance Imaging of the Kidney? Opportunities, Challenges and Future Directions.
Acta Physiol (Oxf). 2014;:ePub - PMID: 25204811
Cerebral blood volume estimation by ferumoxytol-enhanced steady-state MRI at 9.4?T reveals microvascular impact of ?1 -adrenergic receptor antibodies.
NMR in Biomedicine . 2014;27(9):1085-93 - PMID: 25060359
Modulation of dendritic cell immunobiology via inhibition of 3-hydroxy-3-methylglutaryl-CoA (HMG-CoA) reductase.
Ophthalmic magnetic resonance imaging at 7 T using a 6-channel transceiver radiofrequency coil array in healthy subjects and patients with intraocular masses.
Investigative Radiology . 2014;49(5):260-70 - PMID: 24651662
Detailing the Relation Between Renal T2* and Renal Tissue pO2 Using an Integrated Approach of Parametric Magnetic Resonance Imaging and Invasive Physiological Measurements.
Investigative Radiology . 2014;:ePub - PMID: 24651661
Toll-like receptor 2 mediates microglia/brain macrophage MT1-MMP expression and glioma expansion
Neuro-Oncology. 2013;:ePub - EDOC: /13344/
Enlargement of cerebral ventricles as an early indicator of encephalomyelitis
PLoS One. 2013;8 (8):e72841 - EDOC: /13248/
Monitoring dendritic cell migration using 19F / 1H magnetic resonance imaging
Journal of Visualized Experiments. 2013;(73):e50251 - EDOC: /12991/
High temporal resolution parametric MRI monitoring of the initial ischemia/reperfusion phase in experimental acute kidney injury
PLoS One. 2013;8 (2):e57411 - EDOC: /12950/
Visualizing brain inflammation with a shingled-leg radio-frequency head probe for (19)f/(1)h MRI
Scientific Reports. 2013;:1280 - EDOC: /12897/
GDNF mediates glioblastoma-induced microglia attraction but not astrogliosis
Acta Neuropathologica. 2013;:ePub - EDOC: /12810/
High spatial resolution and temporally resolved t(2) (*) mapping of normal human myocardium at 7.0 tesla: an ultrahigh field magnetic resonance feasibility study
PLoS One. 2012;7 (12):e52324 - EDOC: /12746/
Progress and promises of human cardiac magnetic resonance at ultrahigh fields: a physics perspective.
Journal of Magnetic Resonance. 2012;229:208-22 - PMID: 23290625
Functional and morphological cardiac magnetic resonance imaging of mice using a cryogenic quadrature radiofrequency coil
PLoS One. 2012;7 (8):e42383 - EDOC: /12510/
Identification of cellular infiltrates during early stages of brain inflammation with magnetic resonance microscopy
PLoS One. 2012;7 (3):e32796 - EDOC: /12287/
Perfluorocarbon particle size influences magnetic resonance signal and immunological properties of dendritic cells
PLoS One. 2011;6 (7):e21981 - EDOC: /11787/
Understanding the pathogenesis of neuroinflammation using magnetic resonance imaging
Malta Medical Journal. 2011;23 (3):56 - EDOC: /13188/
Oral high-dose atorvastatin treatment in relapsing-remitting multiple sclerosis
PLoS One. 2008;3 (4):e1928 - EDOC: /9481/
Geranylgeranylation but not GTP-loading of Rho GTPases determines T cell function
Science Signaling. 2008;1 (12):pt3 - EDOC: /13905/
Geranylgeranylation but not GTP loading determines rho migratory function in T cells
Journal of Immunology. 2007;179 (9):6024-6032 - EDOC: /8967/