Investigation of Macromolecular Protein Assemblies Using Solid-State NMR Spectroscopy Enhanced by DNP, Proton-Detection and Ultrafast Spinning

Abstract:
Many biologically interesting processes involve proteins or protein assemblies that are attached to or integrated into the cell membrane or other cellular/viral structures which are not dissolved in the cytoplasm. These insoluble proteins are difficult to study by X-ray crystallography or solution-state NMR.
In contrast, solid-state NMR spectroscopy has proven to be a successful technique to determine the structure of such proteins at atomic detail. One of the principal drawbacks of solid-state NMR is its inherently low sensitivity. In this talk, the two most promising approaches for sensitivity enhancement in solid-state NMR (dynamic nuclear polarization and proton detection) together with their applications to macromolecular protein assemblies are presented.

Status: confirmed

PTB research project introduction

Status: confirmed

Photo-chemically induced DNP NMR studies in solution-state with field-cycling and in solid-state

Status: confirmed